Real-Time Imaging of Hepatic Inflammation Using Hydrogen Sulfide-Activatable Second Near-Infrared Luminescent Nanoprobes

The sensing and visualized monitoring of hydrogen sulfide (H₂S) in vivo is crucial to understand its physiological and pathological roles in human health and diseases. Common methods for H₂S detection require the destruction of the biosamples and are not suitable to be applied in vivo. In this Communication, we report a “turn-on” second near-infrared (NIR-II) luminescent approach for sensitive, real-time, and in situ H₂S detection, which is based on the absorption competition between the H₂S-responsive chromophores (compound 1) and the NIR-II luminescent lanthanide nanoparticles. Specifically, the luminescence was suppressed by compound 1 due to the competitive absorption of the incident light. In the presence of H₂S, the compound 1 was bleached to recover the luminescence. Thanks to the deep tissue penetration depth and the low absorbance/scattering on biological samples of the NIR-II nanoprobes, the monitoring of the endogenous H₂S in lipopolysaccharide-induced liver inflammation was achieved, which is unattainable by the conventional histopathological and serological approaches.