Confocal Hyperspectral Microscope
CIMA is a hyperspectral confocal system sensitive between 400 and 1700 nm. Spectral resolution can be as low as 0.2 nm in the visible range, and 0.6 nm in the infrared. It pairs a galvanometer head and one of the fastest and most sensitive cameras on the market to yield an acquisition rate above 300 spectra per second. CIMA provides three acquisition modes: confocal hyperspectral imaging, multispectral fluorescence imaging, and emission spectroscopy of a sample in a cuvette.
CIMA Applications Overview
- Simultaneously study the spatial distribution and spectral properties of complex nanomaterials such as upconverting nanoparticles and quantum dots.
- Perform in vitro or in vivo high resolution hyperspectral microscopy.
- Develop new sensors based on environment dependent spectral shifting of their fluorescence signal.
Product Specifications
VIS | IR | |
---|---|---|
Spectral range | 400-1000 nm | 900-1700 nm |
Spectral resolution (FWHM)* | < 0.2 nm (Custom resolution available upon request) | < 0.6 nm (Custom resolution available upon request) |
Spatial resolution | Diffraction Limited | Diffraction Limited |
Cameras* | Back-illuminated CCD or EMCCD | InGaAs linear array |
Excitation wavelength | 980 nm (other wavelengths available). 3 laser input ports. UHP mercury lamp 130W. | 980 nm (other wavelengths available). 3 laser input ports. UHP mercury lamp 130W. |
Microscope | Scientific grade, inverted | Scientific grade, inverted |
VIS | IR | |
---|---|---|
Scanning region | 300 µm x 300 µm (20X objective), 100 µm x 100 µm (60X objective) | 300 µm x 300 µm (20X objective), 100 µm x 100 µm (60X objective) |
Pinhole diameter | 30 to 200 µm | 30 to 200 µm |
VIS | IR | |
---|---|---|
Cuvette size | Standard 10 mm x 10 mm (3.5 mL) | Standard 10 mm x 10 mm (3.5 mL) |
VIS | IR | |
---|---|---|
Camera | Megapixel colour camera for fluorescence and sample visualization | Megapixel colour camera for fluorescence and sample visualization |
Illumination lamp | UHP mercury lamp 130W | UHP mercury lamp 130W |
Epifluorescence filter cubes | Up to 6 different filter cubes | Up to 6 different filter cubes |
Spec Sheet
Publications
Journal of Visualized ExperimentsCIMA
- Life Sciences
Hyperspectral Imaging as a Tool to Study Optical Anisotropy in Lanthanide-Based Molecular Single Crystals
Inorganic Chemistry
- Life Sciences
Microwave-Assisted Solvothermal Synthesis of Upconverting and Downshifting Rare-Earth-Doped LiYF4 Microparticles
Chemistry a European Journal
- Life Sciences