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NIR hyperspectral microscopy covers the detection range of 900-1700 nm and is ideal for the spatial and spectral identification and measurement of fluorophores that emit in the second biological window. For example, single wall nanotubes (SWNTs) emission bands are narrow (~ 20 nm) and each band corresponds to unique (n, m) species (chiralities). With IR hyperspectral microscopy, it is possible to separate these species, with single SWNT spatial resolution on surfaces, in live cells, and in vivo.

Images obtained by IR hyperspectral microscopy can be used to study fluorescence and spectral heterogeneity from single SWNTs in complex environments, including live cells and tissues.

Locate and identify single SWNTs chiralities

• Identify SWNTs by their IR spectra

• Separate single SWNT (emission band ~ 20 nm)

• Simultaneous imaging of all emitters

• Multiplexing with one laser source


Access to second biological window

• Attenuated tissue absorbance

• Higher depth of penetration

• Less scattering

• Limited autofluorescence

Monitor spectral information

• Changes in intensity of single emitters

• Shifts in wavelength

• Spectral bandwidth variations 


In vivo applications

• In vivo imaging of multiplexed emitters

• In vivo long term sensing

Relevant reading:

Roxbury, D. et al. Hyperspectral Microscopy of Near-Infrared Fluorescence Enables 17-Chirality Carbon Nanotube Imaging. Sci. Rep. 5, 14167; doi: 10.1038/srep14167 (2015).